The invention provides an endothelial progenitor cell composition used for rapid breeding overexpression of VEGF. The endothelial progenitor cell composition used for rapid breeding overexpression ofVEGF comprises an endothelial cell basic medium and an additive; the endothelial cell basic medium is one selected from EGM-2 culture medium, M199, and DMEM; the additive comprises fetal calf serum, fibroblast growth factors, vitamin C, epidermal growth factor, L-glutamine, all-trans retinoic acid, and betamethasone. In culture of endothelial progenitor cells capable of realizing overexperssion ofVEGF, the composition is adopted to maintain the characteristics of the endothelial cells of the endothelial progenitor cells, realizing rapid propagation of cells, and increasing VEGF expression amount.