The invention discloses a fluorescent ink pre-press color simulation method, including the following steps: S1, preparing an original electronic color gamut table that sufficiently contains a common color gamut range and consists of color blocks; S2, selecting a fluorescent ink, a printing machine and a printing condition, using the fluorescent ink and the printing machine to print the original electronic color gamut table on a thin film under the printing condition to obtain a printing color gamut table; S3, illuminating the printing color gamut table with a UV light source in a sealed spaceto ensure that the color of each color block on the printing color gamut table appears, and obtaining an illumination color gamut table; and S4, recording the color of each color block of the illumination color gamut table in a shooting manner, and converting the color into an RGB format to obtain an intermediate electronic color gamut table. According to the scheme of the invention, the fluorescent ink printing effect can be obtained before plate making and printing, and thus the waste of materials and the delay of construction periods can be avoided.