The invention relates to an experimental coke oven for small-scale production of coke and colloid layer samples and a using method thereof. The experimental coke oven comprises a heating furnace, a reactor and a colloid layer sampler; the heating furnace is composed of a furnace shell, a heating furnace body, a carbonization chamber and a top split furnace door which are sequentially arranged fromoutside to inside; heating devices are arranged in the heating furnace body at the two ends of the carbonization chamber respectively; the reactor used for containing coal samples is arranged in thecarbonization chamber and is divided into a main reaction region and a colloid layer sampling region, and the main reaction region and the colloid layer sampling region are respectively close to two heating ends of the heating furnace body; and the colloid layer sampler is movably arranged in the colloid layer sampling region; and the top of the reactor is provided with a reactor upper cover, thereactor upper cover is provided with a gas outlet, a plurality of temperature sensors and a pressure sensor. The experimental coke oven can be used for accurately simulating the working conditions ofa large coke oven, and preparing the colloid layer samples and the coke samples for coking of single coal seed or coal blending; and meanwhile a colloid layer can be sampled at any time, and the experimental coke oven is simple in structure and convenient to operate.