The invention discloses an ACE interval-based multi-type power source active real-time collaborative control method. According to the method, a plurality of types of power sources are divided into priority consumption power sources and non-priority power consumption sources; a strategy that voltage decrease is preferentially performed on non-priority power source commands in a negative emergency region and priority power source commands are preferentially increased in other control regions is adopted to calculate the real-time control commands of the priority power sources and the non-prioritypower sources in a unified manner on the basis of an ACE index, total adjustment power and the interval of the ACE, and therefore, multi-energy joint real-time frequency modulation and peak regulation are realized, the real-time total command of priority consumption power sources such as wind power and photovoltaic power sources can be maximized, the maximized consumption of new energy such as wind and light can be promoted; and multi-source unified and automatic collaborative control also simplifies a real-time control system and greatly reduces the work burden of dispatching operations staff.