The invention provides a making method of a transbronchoscopic lymph node puncture training model using three-dimensional printing. The making method comprises the following steps: firstly, acquiringstructural information of throats, tracheas and bronchi of different persons through CT and three-dimensional reconstruction techniques, and making a plurality of airway models conforming to a real clinical anatomical structure via a three-dimensional printing technique; then, making simulated lymph nodes stuck on corresponding positions of the outer sidewalls of bronchi, a simulated jaw model aswell as a shell for sheltering and fixing a bronchus model; and finally, assembling various parts in accordance with anatomical positions, and covering the outermost layer with the shell. According tothe making method of the transbronchoscopic lymph node puncture training model using three-dimensional printing provided by the invention, the airway structures of the training model are highly similar to the structure of a real case, and the various airway structures in the training model are different in structure and difficulty degree, so that a training process is closer to a real clinical operating environment, and the difficulty of the training process is gradually improved; therefore, the training effect is enhanced.