The invention relates to the field of petrochemical industries, in particular to a low-permeability oil-field oil-well interval-pumping method. The low-permeability oil-field oil-well interval-pumpingmethod comprises the following steps that the formation pressure pe and an oil well inflow dynamic curve QS (p) are obtained; an oil well pump efficiency curve eta (t) is obtained; any point (t, p) on a shut-in well pressure recovery curve Pr (t) serves as the oil well begin-pumping pressure, and the oil well output Q and the visible daily output (Q') within an interval-pumping period are calculated; the visible daily output under different begin-pumping pressure is calculated, the working system during the maximum visible daily output is taken as the optimal interval-pumping system during the stop-pumping pressure p0, the well stopping time is (t-T1), and the well opening time is (T2-t); and different stop-pumping pressure is selected, the maximum visible daily output under a series of stop-pumping pressure is calculated, and the working system corresponding to the maximum value of the visible daily output is the optimal interval-pumping system of an oil well. The low-permeability oil-field oil-well interval-pumping method eliminates the error caused by the fact that the working fluid level converts the bottom hole pressure because the working fluid level has a bubble section.