The invention discloses a plane mirror view field regulation based optical system misalignment resolving method. The method includes implementation steps: establishing an optical system interference check model on the basis of a plane mirror optical path reflection principle; subjecting a detection optical path to view field regulation through regulation of a deflection angle of a plane mirror relative to an ideal position so as to acquire optical system wave aberration under different view fields; establishing a sensitivity matrix on the basis of a wave aberration result, subjecting the sensitivity matrix to pseudo-inverse, and calculating optical system misalignment according to the optical system sensitivity matrix, a wave aberration actual value and a wave aberration ideal value. The method is applicable to plane mirror view field regulation based optical system misalignment resolving, and the problem that establishing of the sensitivity matrix in a traditional resolving process islimited by interferometer onsite regulation in an experiment process is solved.