The invention relates to the technical field of culture boxes, in particular to a culture box capable of fixing culture dishes in different specifications. The top of a box body is not sealed, air holes are formed in the left side face and the right side face of the box body, and sliding grooves are formed in the left side and the right side of the interior of a box cover; the box cover is slidably sleeved with the sliding grooves, and grooves are formed in the left side face and the right side face of the box body from top to bottom; a baffle is sleeved with the grooves and is rotationally hinged to the box body; trays are arranged in the box body, a supporting column is fixedly arranged at the bottom of each tray, the supporting columns are fixedly connected with the box body, and a sleeve is fixedly arranged on the lower surface of each tray; first fixing blocks are slidably sleeved with each sleeve, and first fixing plates are arranged on the two sides of each sleeve, and the two ends of each first fixing block are fixedly provided with second fixing plates, and first springs are arranged between the first fixing plates and the second fixing plates; second sleeves are fixedly arranged at the tail ends of the first fixing blocks, second fixing blocks are slidably sleeved with the second sleeves, and second springs are fixedly arranged at the bottoms of the second fixing blocks.