The invention relates to a biological material 3D printer multi-inlet one-outlet sprayer. The sprayer comprises a cylinder on the upper portion and a gradually-varied cone at the lower portion of thecylinder, a plurality of feeding holes are evenly distributed in the cylinder along the circumference, the side face of the cylinder is provided with an installing hole for fixing the sprayer, a discharging hole is formed in the center of the cone, each feeding hole comprises a straight line section and an inclined transition section, the feeding holes are communicated with the discharging hole through the inclined transition sections, and the feeding holes and the discharging hole are independent and are not communicated at the communication positions; a spraying nozzle is connected to the lower end of the discharging hole; and the printer sprayer is made of titanium alloys. The sprayer is of a multi-inlet one-outlet structure, and the problems that when an existing biological material 3Dprinter is used for printing multiple kinds of materials, a plurality of needle barrels are needed, printing at the joining position is not stable, and materials are not continuous are solved.