The invention discloses a preparation method of a silver catalyst using amino acid as a guiding agent to prepare 3,4-epoxybutene via 1,3-butadiene. The preparation method comprises: adding certain load guiding agent using amino acid as a silver active component and alkaline metals and/or alkaline-earth metals and/or transition metals and the like as active aids during the preparation, so that theactive components such as silver form a membrane having uniform protrusions on the surface of alpha-Al2O3. The silver catalyst has the advantages of good activity, high selectivity, good stability andthe like. The membrane distribution of the active components, elements such as silver, in the silver catalyst can be imparted membrane distribution without making complex changes to the existing preparation processes and means. The method is simple to perform, low in cost, good in controllability and good in preparation convenience and meets the requirement for high selectivity and significantlyimproved stability at the premise of high activity.