The invention discloses an explosion-proof membrane clamping device which comprises a first installing block. Two rotating rods are symmetrically arranged on the first installing block, one ends of the two rotating rods penetrate the side wall of the first installing block to be connected with a rotating handle, first gears are inserted into the other ends of the rotating rods, second gears are connected to one ends of the first gears in a meshed manner, first rotating shafts are inserted into the second gears, and threaded blocks are in threaded connection to the first rotating shafts. The explosion-proof membrane clamping device is simple in structure and easy to operate; through cooperation of structures such as a first cylinder pin, a second rotating shaft, a ring plate, a connecting rod and a special-shaped rotating wheel, an explosion-proof membrane body can be pressed through a pressing plate, and dismounting and mounting are facilitated; and through cooperation of structures such as a connecting plate, a second spring and a third installing groove, the distance between a first clamping plate and a second clamping plate can be changed, the size of a fourth installing grooveis changed, samples in different sizes can be tested, and the accuracy of a test result is increased.