The invention discloses a pipeline for connecting a laser and a vacuum environment. The pipeline comprises a stainless steel pipeline and a second oxygen-free copper pipe. A first oxygen-free copper pipe, a three-way joint, a ceramic pipeline and a straight joint are connected between the stainless steel pipeline and the second oxygen-free copper pipe. A getter supply device is connected with theside joint of the three-way joint. The invention further provides the manufacturing method and the application of the pipeline. According to the invention, after the connection of the pipeline is completed, the leakage rate can be better than 5.0*10-13 Pa.m<3>/s at any temperature in the range of minus 40 DEG C to 135 DEG C; the pipeline can meet the process requirement of long-time baking degassing; the pipeline can use a cold sealing clamp to cold-seal an oxygen-free copper pipe; during the cold sealing process, the gas in the laser is not exchanged with the external environment, which ensures that the purity of the working gas in the laser does not change; other processes can be carried out on a device composed of the cold-sealed laser and the getter supply device, and then second coldsealing is carried out; and a high-precision laser product is acquired after the getter supply device is removed.