The invention provides a pixel circuit driving method, a display panel and a display apparatus. The method herein includes the following steps: dividing a pixel circuit to a plurality of repetition units, decomposing an image having X hertz to two images of 2X hertz, in a first frame of time, a first part pixel in each repetition unit normally displaying, a second part pixel displaying a referencecolor; in a second frame of time, the second part pixel in each repetition unit normally displaying, the first part pixel displaying the reference color; the first part pixel and the second part pixel are alternatively arranged in the repetition unit, such that the adjacent pixels alternatively display a normal color and the reference color and uneven laser annealing is reduced. Therefore, the display effects of the display apparatus are increased.