The invention discloses a 7-carbonyl-tauroursodeoxycholic acid crystalline compound for the first time and a preparation method thereof. The crystalline compound disclosed by the invention is determined through a powder X-ray diffractometry and an X-ray powder diffraction pattern shown by a 2theta+/-0.2 degree diffraction angle has haracteristic diffraction peaks when the angles are 5.1 degrees, 10.1 degrees, 13.8 degrees, 14.3 degrees, 15.1 degrees, 17.6 degrees, 18.0 degrees, 18.3 degrees, 19.0 degrees, 21.0 degrees, 22.3 degrees, 23.2 degrees, 24.2 degrees, 24.9 degrees, 25.6 degrees, 27.4degrees, 29.3 degrees, 30.8 degrees, 32.3 degrees, 34.0 degrees, 36.1 degrees and 37.5 degrees. According to the 7-carbonyl-tauroursodeoxycholic acid crystalline compound and the preparation method thereof, a target crystallized product is obtained through three steps of reacting through a one-pot method, extraction purifying and recrystallizing; the preparation method has the advantages of low cost, easiness for obtaining raw materials, simplicity and convenience for operation and short reaction period; a test proves that the crystalline compound provided by the invention has relatively goodcrystal form stability in a storage process and a preparation process.