The invention discloses a microenvironment cooling system control method based on human body thermal load dynamic response. The method comprises the following steps that (1) after a microenvironment cooling system is started to run, environment temperature, relative humidity, airflow velocity, cooling system outlet refrigeration liquid temperature and cooling system inlet refrigeration liquid temperature are collected; (2) the comfortable degree of human body SSD is calculated in real time; (3) the SSD stage is determined according to the obtained comfortable degree of the human body, the cooling system refrigeration liquid initial output liquid temperature is set according to the determined SSD stage, and after stable running is carried out for time t, the cooling system inlet and outletrefrigeration liquid temperature average difference value within the time t is calculated according to the detected outlet refrigeration liquid temperature and the inlet refrigeration liquid temperature; and (6) every time t, the cooling system output refrigeration liquid temperature of a next stage is automatically adjusted. According to the microenvironment cooling system control method, the cooling system output liquid temperature is dynamically adjusted, the more comfortable microenvironment is provided for a user, and the using comfort of the liquid cooling type microenvironment cooling system is improved.