The invention discloses an antibacterial silk product with nano-copper and a preparation method of the antibacterial silk product with nano-copper. The preparation method comprises the following steps: immersing a silk product in a buffer solution containing monascus, then adding a copper hydrochloride solution for soaking the immersed silk product, then performing reduction treatment with a reducing agent, and finally, performing cleaning and drying to obtain the antibacterial silk product with nano-copper. The antibacterial silk product with nano-copper and the preparation method thereof have the advantages that the broad-spectrum antibacterial property of silk fibers and fabrics thereof can be improved, furthermore, harm to human bodies can be avoided, and pollution to the environment also can be avoided; the preparation method is simple and easy to realize batch production; and the prepared antibacterial silk fiber product has excellent and lasting antibacterial property.