Disclosed is an organ chip unit containing pipeline networks. The unit comprises one or more chips containing input pipeline networks, one or more chips containing output pipeline networks, one or more chamber chips, at least two microporous membranes, at least two barrier cells and two encapsulation plates; the input pipeline networks are used for inputting, diverting and diffusing substances; the output pipeline networks are used for diffusing, converging and outputting the substances; the chamber chips contain parenchymal organ tissue; the parenchymal organ tissue is separated from the input and output pipeline networks by the microporous membranes; the barrier cells are located in the input and output pipeline networks and adhere to the microporous membranes; the two encapsulation plates are used for achieving encapsulation. By using the organ chip unit, the process can be simulated that the substances are diverted through various pipeline networks such as vascular/lymphatic/bronchial/glandular secretion networks, then enter the tissue and are subjected to metabolism in the tissue and gradually converged, the advantages of low cost and rapid performance of the chip are ensured,and meanwhile the organ chip unit is more accurate when applied to medicine screening, case studies and physiological analysis.