The invention discloses an automatic needle tube sorting machine, which comprises a guiding slot, a rotary roller and a conveying device. The slot length is equal from top to bottom; the slot width ofa lower end slot port is matched with the diameters of target needle tubes, so that the lower sides of the target needle tubes are exposed out of the lower end slot port under a gravity action; the rotary roller is arranged under the lower end slot port and extends along a slot length direction of the guiding slot; needle holding slots extending along an axial direction of the rotary roller are arranged on the outer side of the rotary roller; when slot walls of the needle holding slots rotate to the lower end slot port, the target needle tubes exposed out of the lower end slot port can be pushed to be separated from the lower end slot port and enter the needle holding slots; and the conveying device is arranged under the rotary roller and is used for conveying the target needle tubes to asorting part. The needle tubes are separated one by one through the rotary roller and the guiding slot, so that the automatic needle tube sorting machine can be used for effectively solving the problem that a large number of target needle tubes are difficult to separate one by one.