The invention discloses a catalyst for desulphurization of hydrocarbon oil and a preparation method thereof, a method for desulphurization of hydrocarbon oil, belonging to the field of desulphurization of hydrocarbon oil. On the basis of the total weight of the catalyst, the catalyst comprises 1) 10 to 80 wt% of at least one selected from metal oxides of group-IIB elements, group-VB elements and group-VIB elements; 2) 3 to 35 wt% of alumina; 3) 5 to 40 wt% of vanadium carbide; 4) 1 to 20 wt% of a SAPO molecular sieve; and 5) 5 to 30 wt% of a metal promoter, which is at least one selected froma group consisting of cobalt, nickel, iron and manganese. The catalyst for desulphurization of hydrocarbon oil has better stability, higher desulphurization activity, better wear resistance and longerservice life and is capable of obviously increasing the octane number of gasoline.