The invention provides a buffer layer of a functional shoe sole. The buffer layer is prepared from, by weight, 85-105 parts of polyisoprene, 9-15 parts of cyclohexyl thiomaleimide, 6-11 parts of thiuram disulfide, 8-17 parts of 1,4-diamino-butane, 16-25 parts of polyacrylamide, 23-29 parts of polystyrene nanoparticles, 31-45 parts of polylactic acid fiber, 8-15 parts of silicate acetate and 11-23parts of kaoline. Due to synergic cooperation of the components, the damping performance and wearing comfort of the shoe sole are greatly improved, and the shoe body is easier and attractive in wholeshape and capable of meeting the requirement of the public easily.