A method for producing a surface-directed imprinted polymer-modified quartz crystal microbalance sensor is characterized in that a surface-directed imprinted polymer is obtained in a reverse micro-emulsion manner with the carbon terminal octapeptide of palmitic acid modified cytochrome C as a template, and the surface of a quartz crystal microbalance is modified with the surface-directed imprintedpolymer to obtain the sensor. The method comprises the following steps: preparing an organic phase; dropwise adding a water phase into the organic phase to prepare a reverse micro-emulsion system; preparing the molecularly imprinted polymer; and producing the molecularly imprinted polymer-modified quartz crystal microbalance sensor. The method has the following advantages: a palmitic acid modified antigenic determinant is directionally imprinted on the surface of a polymer during reverse micro-emulsion polymerization; the imprinted polymer synthesized through an antigenic determinant technology can selectively identify target cytochrome 3 protein (the imprinting factor is 3); and the antigenic determinant imprinted polymer-modified quartz crystal microbalance sensor can selectively determines cytochrome C having a low concentration (5-50 ng/mL).