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CN107937489A 一种基于RAA荧光法检测十二指肠钩虫的引物、试剂盒 无效 - 驳回

一种基于RAA荧光法检测十二指肠钩虫的引物、试剂盒
摘要：
本发明提供了一种基于RAA荧光法检测十二指肠钩虫的引物、试剂盒，属于分子生物学技术领域。所述引物包括上游引物和下游引物，所述上游引物具有如序列表中SEQ ID No.1所示的核苷酸序列；所述下游引物具有如序列表中SEQ ID No.2所示的核苷酸序列。本发明提供的引物适用于RAA荧光法检测，并且能够准确地检测出十二指肠钩虫，特异性达100％。本发明还提供了一种基于RAA荧光法检测十二指肠钩虫的试剂盒，所述试剂盒方便准确地鉴定十二指肠钩虫的存在，仅需5～20min即可完成，无需通过高温使DNA解旋，在30～42℃下进行等温扩增即可完成检测。
摘要（英）：

The invention provides an ancylostoma duodenale primer and a ancylostoma duodenale kit based on RAA fluorescence method detection and belongs to the technical field of molecular biology. The primer isprepared from an upstream primer and a downstream primer, wherein the upstream primer has a nucleotide sequence showed by a SEQ ID No.1, and the downstream primer has a nucleotide sequence shown by aSEQ ID No.2. The primer disclosed by the invention is suitable for the RAA fluorescence method detection and further can accurately detect ancylostoma duodenale, and specificity can reach 100%. The invention further provides the ancylostoma duodenale kit based on the RAA fluorescence method detection. The kit can conveniently and accurately identify existence of the ancylostoma duodenale, identification can be finished in 5 to 20min, high-temperature DNA despiralization is avoided, and isothermal amplification is performed under 30 to 42 DEG C to finish detection.

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