The invention discloses a stress response analysis device and method based on eye movement characteristics of a driver. The stress response analysis device includes an automobile driving simulator, aneye tracker, an alarm unit, a data processing unit and a comprehensive analysis unit. The automobile driving simulator, the eye tracker and the alarm unit are connected to the data processing unit, and the data processing unit is connected to the comprehensive analysis unit. By arrangement of the automobile driving simulator, the eye tracker, the alarm unit, the data processing unit and the comprehensive analysis unit, data processing is performed on the driver's stress response perception, decision and operation, stress response time characteristics of the driver are analyzed, and the theoretical support is provided for the improvement of driving behavior safety.