The invention provides an ACPI test method and system for a network card, and a test machine. The method comprises the steps that S10, the test machine configures the to-be-tested network card; S20, the test machine changes a power mode to a non working power mode according to a received test instruction and then tests a working state of the to-be-tested network card in the non working power mode;S30, the test machine switches the changed power mode to a working power mode according to the receiving test instruction and then tests a working state of the to-be-tested network card in the working power mode; S40, when the to-be-tested network card is tested in the power mode of a non working power mode class, a test result is fed back to a host, or otherwise, the operation of returning to the step S20 is performed for retesting; and S60, the test instruction resent by the host according to the test result is received, and the operation of returning to the step S20 is performed for testing the working state of the to-be-tested network card in another untested power mode of non working modes. The network card can be automatically tested.