The invention provides a non-woven fabric wet wipe grafted with acrylamide and organic silicon biquaternary ammonium salt. The non-woven fabric wet wipe comprises a wet wipe body and wet wipe liquid,wherein the wet wipe body is a non-woven fabric bonded with macromolecular quaternary ammonium salt; the wet wipe liquid adopts RO purified water, 12-18 alkyl glucoside, lecithin or hydrogenated lecithin, 1,2-pentanediol, a skin conditioner and other additives, 0.1-1.0 mg/L ozone concentration is introduced into the wet wipe liquid, finally, the wet wipe liquid is added to the wet wipe body at a certain weight ratio, sealed packaging is carried out, and thus the non-woven fabric wet wipe grafted with acrylamide and organic silicon biquaternary ammonium salt is formed. The product belongs to the wet wipe with zero separation of a preservative, is mild to the skin, is free of stimulation and irritability to the skin, and is particularly suitable for the skin types including the allergic skin, the tender skin and the like.