The invention relates to a preparation method for a sheet-stacked molybdenum disulfide nanotube. The preparation method comprises the following steps: dissolving a molybdenum source and sodium chloride in a solvent, carrying out a hydrothermal reaction under stirring, then carrying out cooling, vacuum filtering, washing and drying so as to obtain a molybdenum oxide nanobelt, mixing the molybdenumoxide nanobelt with a sulfur source, carrying out grinding, then carrying out vulcanizing under protective gas, and carrying out washing, vacuum filtering and drying. The preparation method provided by the invention is simple and highly-efficient, facilitates operation, is an effective and rapid preparation method, and has the prospect of large-scale preparation; and the sheet-stacked molybdenum disulfide nanotube prepared by using the preparation method provided by the invention has a structure that molybdenum disulfide is shown as an ultrathin nanosheet and can be self-assembled into a three-dimensional nanotube, and the structure facilitates complete exposure of active sites of molybdenum disulfide.