The invention provides a multi-frequency reconfigurable antenna with the sequential alternating of left-handed and right-handed circular polarization, and belongs to the technical field of microwave antennas. The antenna consists of 16 radiation units, and the radiation units are distributed in the shape like the Chinese character Mi. Each four radiation units serve as one group, and work at the same frequency band. There are four working frequency bands, and the antenna can be extended to more working frequency bands according to the invention. Four radiation units working at the same frequency band jointly achieve the circular polarization in one direction, and the directions of the circular polarization of each two adjacent frequency bands are opposite. According to the scheme of the invention, the antenna can achieve the circular polarization in the opposite directions at the adjacent working frequency bands, and can further achieve the reconfiguration of the left-handed and right-handed circular polarization through the introduction of a switch. The antenna can be used in a satellite communication system, a civil communication system and other microwave communication systems.