The invention provides a method for sealing a glass-base microfluidic channel by virtue of a glass direct bonding process. The method comprises the following steps: carrying out surface activation onpolished and cleaned glass in an H2SO4:H2O2 mixed solution, putting the glass into an NH4OH:H2O2:H2O mixed solution, and cleaning; (2) rapidly aligning, and carrying out finger pressing for several minutes; and (3) putting samples into a furnace, adding a pressure block with a pressure of 18kp-28kp, heating to a glass softening point, maintaining accessories for 1-2 hours, and slowly cooling to the room temperature, so as to finish the sealing. By virtue of good polishing, the gap width of the glass is reduced; by virtue of surface activation, the surface of the glass is endowed with a hydrophilic property; and by aligning, the contact surface is increased, the discharging of bubbles is promoted, and an electrostatic field and a Van der Waals' force are enhanced. By virtue of thermal treatment, the glass is in a viscous and elastic state, the surface bond energy of the glass is increased, and meanwhile, a micro-channel structure is stable; and by utilizing a slow cooling manner, the bonding thermal stress of the glass is reduced. The method has the beneficial effects that the glass is high in binding force, the service life of a chip is long, and a micro-channel is well maintainedand is not deformed, blocked and collapsed.