The present invention discloses a cold-storage and preservation method of cold shock treated and chlorogenic acid negative pressure treated nectarine fruits. The cold-storage and preservation method is characterized by comprising the following steps: surfaces of mature nectarine fruits are firstly cleaned with a microbicide, then the nectarine fruits are soaked with gibberellin, and then the nectarine fruits are soaked with salicylic acid; the treated nectarines are soaked with carbendazim, the soaked nectarines are air-dried with cold air as a medium, and the nectarines are subjected to a cold shock treatment; the obtained nectarine fruits are soaked in a chlorogenic acid solution, the soaked nectarine fruits are treated under negative pressure, and the treated nectarine fruits are air-dried at room temperature; and ClO<2> solid powder is dissolved in water to form a solution, the nectarine fruits are soaked in the solution, a mouth of a container is sealed with a plastic film, adhered water on the treated nectarine fruits are air-dried, then the air-dried nectarine fruits are fumigated at room temperature using 1-methylcyclopropylene, then the nectarine fruits are ventilated, theventilated nectarine fruits are packaged in perforated polyethylene film bags, and the packaged nectarine fruits are stored in a mechanical cold storage.