The invention relates to a rapid gel electrophoresis and real-time imaging device. The device can realize rapid gel electrophoresis, can be used for monitoring the electrophoresis process in real time, and is suitable for the study in the fields such as clinical disease diagnosis and molecular biology. The device mainly consists of a support system, an electrophoresis system, a light source, a voltage control system and an imaging system. An electrophoresis chip (with an authorization announcement number of CN206591103 U) is put into a support module and is fixed; then, electrode wires are placed into an electrode slot of the chip by means of a support pillar of an electrophoresis module, and the voltage between the two electrode wires is controlled by means of a voltage control module; therefore, rapid gel electrophoresis of biomolecules, such as deoxyribonucleic acid (DNA), ribonucleic acid (RNA) or protein, to be measured can be realized; the light source consists of light-emittingdiode (LED) arrays, and can excite a conjugate of a sample to be measured and fluorescent dye in the chip after passing through a light filter, so that emitted light is produced; another light filterarranged at the upper part is used for filtering stray light, and finally, the electrophoresis process is monitored in real time by means of the imaging system. The rapid gel electrophoresis and real-time imaging device has the characteristics of miniaturization, low cost, high efficiency, simple operation, and the like.