The invention provides a preparation method of an adipose-derived stem cell micro-vesicle loaded biotinylated titanium plate. The preparation method comprises the following main steps: (1) pre-treating a titanium plate; (2) preparing a biotinylated polypyrrole (Biotin-Ppy) titanium plate; (3) preparing a biotinylated adipose-derived stem cell micro-vesicle (ASCs-MV); (4) carrying out compound inoculation on the biotin marked ASCs-MV and the biotinylated polypyrrole titanium plate to prepare the adipose-derived stem cell micro-vesicle loaded biotinylated titanium plate. According to the preparation method provided by the invention, the ASCs-MV has good vessel regeneration promotion and stem cell proliferation promotion performance; an avidin-biotin system with a cascade amplification effectis utilized and the ASCs-MV can be successfully used for carrying out surface modification on the biotinylated polypyrrole titanium plate, so that bone formation and blood vessel formation capabilities of osteoblasts are effectively improved and a potential material modification material is provided for bone regeneration.