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发明授权
CN107831208B 纳米复合材料、SDM电化学适体传感器的制备方法及检测方法 失效 - 权利终止

纳米复合材料、SDM电化学适体传感器的制备方法及检测方法
摘要：
本发明公开了一种P‑rGO‑C60纳米复合材料和P‑rGO‑C60‑Pt@Au纳米复合材料，还公开了一种用于SDM检测的电化学DNA适体传感器，由以下方法制备得到：1)用TCEP溶液处理SDM适体备用；2)将玻碳电极抛光成镜面，处理电极，干燥备用；3)将电极电化学清洗，水冲洗，干燥；4)将6～10μLP‑rGO‑C60溶液滴加到玻碳电极表面上，干燥，将GOD溶液滴加到电极上；5)将P‑rGO‑C60‑Pt@Au溶液滴加到电极表面，室温干燥；6)取SDM结合适体滴加在电极上室温孵育；7)将电极浸入GOD溶液，即得。还公开了采用该传感器检测SDM的方法。
摘要（英）：

The invention discloses a P-rGO-C60 nanocomposite material and a P-rGO-C60-Pt@Au nanocomposite material, and also discloses an electrochemical DNA aptamer sensor for SDM detection, wherein the electrochemical DNA aptamer sensor is prepared by the following method: 1) treating an SDM aptamer by using a TCEP solution for standby application; 2) polishing a glassy carbon electrode into a mirror surface, processing the electrode, and drying for standby application; 3) electrochemically cleaning the electrode, swashing with water, and drying; 4) dropwise adding 6-10 [mu]L of P-rGO-C60 solution intothe surface of the glassy carbon electrode, drying, dropwise adding a GOD solution to the electrode; 5) dropwise adding the P-rGO-C60-Pt@Au solution to the electrode surface, and drying at room temperature; 6) taking an SDM binding aptamer, dropwise adding on the electrode, and incubating at room temperature; and 7) immersing the electrode in the GOD solution, and thus obtaining the product. A method for detecting the SDM by using the sensor is also disclosed.

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