The invention provides a support frame for time-resolution fluorescence testing. The support frame comprises a sample container, a sample container fixing groove, a support sheet, an angle regulatingshaft, a fixing duct, an exciting-light filtering piece clamping groove and an emitting-light filtering piece clamping groove. The sample container is used for holding samples. The sample container fixing groove is used for fixing the sample container. The support sheet and the angle regulating shaft control the angles between the samples, exciting light as well as emitting light and a detection light path jointly. A sample platform duct and a fixing screw are used for replacing and fixing different instrument sample pools. The exciting-light filtering piece clamping groove and the emitting-light filtering piece clamping groove are used for holding filtering pieces with different wavelengths, interference of stray light is weakened or eliminated, and the signal to noise ratio of testing isincreased. The support frame for fluorescence testing has a universal type, can control the relative positions between the samples and the testing light path through an external shaft, and is particularly applicable to time-resolution fluorescence testing of colloidal solutions and solid or film samples.