知搜-全球专利检索

  1. 登录
  2. 注册
发明公开
CN107817339A 壳聚糖修饰的AgI/TiO2复合物及其构建比色免疫传感器用于氯霉素检测的方法 无效 - 驳回

壳聚糖修饰的AgI/TiO2复合物及其构建比色免疫传感器用于氯霉素检测的方法
摘要：
本发明涉及食品中氯霉素检测领域，具体是制备壳聚糖修饰的AgI/TiO2复合物，利用其光催化性质构建比色免疫传感器，实现对氯霉素的检测。首先制作壳聚糖修饰的AgI/TiO2复合物，然后将CAP-BSA/MB依次孵育不同浓度的氯霉素和anti-CAP-CS-AgI/TiO2，然后用水清洗2-5次，构建成免疫传感器，随后加入ABS缓冲液和四甲基联苯胺TMB溶液，利用氙灯即波长λ ≥ 400的光照射5分钟，通过紫外分光光度计检测TMB的紫外吸收来定量氯霉素的浓度。
摘要（英）：

The invention relates to the field of detection of chloramphenicol in food, in particular to preparation of a chitosan modified AgI/TiO2 compound, construction of a colorimetric immunosensor by utilizing photocatalytic properties of the compound and realization of detection on chloramphenicol. According to the invention, firstly the chitosan modified AgI/TiO2 compound is produced, then CAP-BSA/MBis used for successively hatching different concentrations of chloramphenicol and anti-CAP-CS-AgI/TiO2, then washing is carried out with water for 2-5 times, the immunosensor is constructed, then ABSbuffer solution and tetramethyl benzidine (TMB) solution are added, a xenon lamp, namely light with the wavelength lambda more than or equal to 400, is utilized for irradiating for 5 minutes, and chloramphenicol concentration is quantified by detecting ultraviolet absorption of TMB by virtue of an ultraviolet spectrophotometer.

0/0