The invention provides a red steppe cattle beef quality-related molecular marker and application thereof in meat quality identification and belongs to the fields of biotechnology and crop and pastureproduction. The red steppe cattle beef quality-related molecular marker provided by the invention is closely related to meat quality of red steppe cattle, and when the red steppe cattle beef quality-related molecular marker is applied to the meat quality identification of red steppe cattle, beef with higher quality can be better distinguished and screened out, so that the red steppe cattle beef quality-related molecular marker can better guide production of agriculture and animal husbandry, especially the animal husbandry, and has greater use value and social value.