The invention provides a molecular marker relevant to grassland red cattle physical signs and application thereof, and belongs to the field of biotechnology and crop and pasture production. The molecular marker relevant to grassland red cattle physical signs provided by the invention is closely relevant to the beef quality of the grassland red cattle. When the molecular marker relevant to grassland red cattle physical signs is applied to the grassland red cattle beef quality analysis and identification; high beef-quality beef can be well identified and screened out, so that the molecular marker relevant to grassland red cattle physical signs can be used for better guiding the production of crop and pasture, particularly the animal husbandry. Great use values and social values are realized.