The invention relates to the technical field of biology and discloses a beta-agonist drug test strip and application thereof. A preparation method includes: preparing a conjugate release pad coated with beta-agonist drug monoclonal antibody-colloidal gold marker; preparing a detection line coated with beta-agonist drug hapten-carrier protein conjugate and a quality control line coated with goat anti-mouse antiantibody; assembling the prepared conjugate release pad, a reaction film, a sample absorption pad, a water absorption pad and a base plate to form the test strip. The beta-agonist drug test strip has advantages of simplicity in operation, high sensitivity, high test speed, low cost and freeness of detection equipment limitation, and onsite monitoring and quick testing of large-batch samples are realized.