The invention provides a spiral soft tissue biopsy needle which comprises a puncture needle rod (1), a sampling needle rod (2) and a handle (3) for fixing the puncture needle rod (1) and the samplingneedle rod (2). The sampling needle rod (2) comprises a spiral needle rod (21) for fixing a sampled part and a cutting needle rod (22) for separating a sample from the sampled part, the spiral needlerod (21) is of a hollow structure, a spring helix tube (211) is arranged at the front end of the spiral needle rod (21), and a cutting edge (221) is arranged at the front end of the cutting needle rod(22). The spiral soft tissue biopsy needle can improve one-time sampling success rate and achieve quantitative sampling, meanwhile can achieve multi-point continuous sampling, and meanwhile the success rate of larger samples can be improved or whole taking out of small tumors and hyperplasia parts can be achieved through an end cutting knife (9).