The invention relates to the technical field of testing devices, in particular to a device for testing the service life of a mouse wheel. The device includes a frame, a horizontal placement surface isarranged above the frame, and a horizontal rotating table is arranged above the placement surface. The rotating table can freely rotate, and the height can be adjusted and fixed. A press testing device and a rotation testing device are arranged above the placement surface and fixedly connected with the rotating table. The press testing device includes a press wheel, a first micro motor is arranged on the rotating table, the rotation testing device includes two clamping parts, and the two clamping parts form a spring-clip-like structure through a torsion spring; the clamping ends f the two clamping parts are provided with two rubber wheels respectively, and second micro motors are arranged on the clamping parts. Two mice are placed on the placement surface, and the test parts of the mice are connected with the press testing device and the rotation testing device so as to achieve the test function. The rotating table can be rotated for testing in sequence. The device is simple in structure and wide in applicability.