The invention discloses a liquid mixing and uniform shaking device for preparation of a glucose solution for injection. The liquid mixing and uniform shaking device comprises a base, a first drive motor, a material mixing box, a filtering box and a second drive motor; a joint bearing is rotationally mounted on a rotating base, and a spherical surface on the upper side of the joint bearing is fixedly mounted at the bottom of a fixed seat; material distribution wheels are rotationally mounted under a first feeding opening and a second feeding opening through rotating shafts; the filtering box ismounted on a slide bar in a sliding manner; a cylinder output shaft is horizontally connected with the filtering box leftwards. The liquid mixing and uniform shaking device disclosed by the inventionhas the beneficial effects that as the material distribution wheels are arranged in the liquid mixing and uniform shaking device, and material storage cavities in the material distribution wheels areconstant in volume, interval quantitative feeding can be realized, so that the excessive solution addition from any side is avoided. Therefore, the concentration of the prepared glucose solution is ensured to meet the requirements; meanwhile, after being mixed, the glucose solution can be uniformly shook again, so that the mixing degree of the glucose solution is improved, and the situation of layering due to long-time placement is avoided.