The invention discloses a detection system and a detection method of foot and limb disease of a donkey. The system comprises a center of gravity sensing chip, wherein the center of gravity sensing chip is connected with a signal converter, the signal converter is connected with a first signal transmitter, the first signal transmitter is connected with a first signal receiver, the first signal receiver is connected with a controller, the controller is connected with an electric wave converter, the electric wave converter is connected with a waveform comparator, the waveform comparator is connected with a second signal transmitter, the second signal transmitter is connected with a second signal receiver, the second signal receiver is connected with a number processor, the number processor isconnected with the signal converter, and the controller, the center of gravity sensing chip, the signal converter and the first signal transmitter are connected with a power supply device. The detection system and the detection method have the advantages of finding and curing the foot and limb disease of the donkey timely.