The invention provides a self-targeted anti-cancer nano-particle. The self-targeted anti-cancer nano-particle is formed by connecting a surface carboxylated metal oxide and an active part through an amido bond; the active part is acetylated hyperbranched polyethyleneimine which is bonded with Raltitrexed; the active part has the mass content of 10 to 30 percent; the acetylated hyperbranched polyethyleneimine is connected with the Raltitrexed through the amido bond; the acetylated hyperbranched polyethyleneimine and the Raltitrexed are in the molar ratio of 1:1-8. The self-targeted anti-cancernano-particle is designed by utilizing multivalent interaction; the self-targeted anti-cancer nano-particle is loaded with a plurality of Raltitrexeds to form a multivalent system; high selectivity ofcancer cells is realized through strong bonding force of the multivalent system and a receptor; meanwhile, the toxicity of the Raltitrexed is utilized to specifically kill the cancer cells.