The invention provides a method for inducing the formation of flower buds in a dendrocalamus barbatus test tube. The method comprises the steps of selecting dendrocalamus barbatus sterile clustered vegetative buds, inoculating the vegetative buds to a flower bud induction culture medium, carrying out subculture once every 21 days, and carrying out three times of continuous flower bud induction culture and one time of recovery culture, so as to form the flower buds in the about 85th day. The method provided by the invention has the advantages that the operation is simple, the consumed time is short, the induction efficiency is high, and the repeatability is good; and a research material is provided for the prediction of the flowering of woody bamboo plants and the regulation and control offlowering time, and scientific guidance is provided for the decrease of the economic loss in the management of a bamboo forest.