The invention relates to a semi-automatic culture bottle washing process, which is characterized by comprising the following steps: step S01, forming a limit groove for placing a culture bottle on a conveyer belt, placing the culture bottle to be cleaned in the limit groove; step S02, arranging a culture bottle washing device comprising a washing component and a water wiping component at the end of the conveyer belt, and sequentially completing step S021 and step S022 by use of the two components, wherein the step S021 includes removing the culture bottle from the conveyer belt and placing theculture bottle upside-down onto a brush of the washing component for water-jet washing, and the step S022 includes placing the washed culture bottle upside-down onto a water-absorbing sponge of the water wiping component for wiping the water; and step S03: manually taking out the culture bottle and fitting it into a culture bottle storage box. The process can clean the culture bottle with high washing efficiency.