The invention relates to the fields of molecular biology and bioinformatics, and discloses a method for assessing an attacking risk of Alzheimer's disease based on sanger sequencing (or called the first generation of sequencing). The method is characterized in that by conducting sequencing on related gene APOE of the Alzheimer's disease, the genotype of a site, which is related to the Alzheimer'sdisease, on the gene is determined; and in the combination with a bioinformatics algorithm, gene background support is offered to assess the attacking risk of the Alzheimer's disease. With the application of the method provided by the invention, the attacking risk of the Alzheimer's disease can be simply, conveniently and rapidly assessed in a qualitative mode, so that purposes of predicting and preventing the disease (the Alzheimer's disease) can be achieved.