The invention provides an anti-hair loss, hair-reinforcing and hair-growing composition, a preparation method and application thereof. The anti-hair loss, hair-reinforcing and hair-growing composition is prepared from fullerene, base oil and essential oil. The anti-hair loss, hair-reinforcing and hair-growing composition has the advantages that the medicine effect is stable, the safety in use is realized, the composition can be easily stored and be absorbed by skin, the health of hair follicles is effectively maintained, the injured hair follicles are activated, the inflammation is eliminated, the injured hair follicles are recovered, and the comprehensive effect of preventing hair loss, reinforcing hairs and growing hairs for a long time is realized.