The invention discloses a preparation and automatic collection method for continuous ultrathin sections. The method comprises the steps of: preparing continuous ultrathin sections by a slicing machine; according to the slicing speed of the slicing machine, adjusting the collection speed of an ultrathin section collector; automatically collecting the continuous ultrathin sections prepared by the slicing machine through the ultrathin section collector; during adjustment of the collection speed of the ultrathin section collector, conducting real-time image acquisition on a collection belt of the ultrathin section collector and ultrathin sections on the collection belt, and analyzing the positions of the ultrathin sections and holes on the collection belt, then manually or automatically adjust the slicing speed of the slicing machine, and automatically adjusting the collection speed of the ultrathin section collector in real time in the whole collection process. The method can greatly expand the applicability and diversity of the continuous ultrathin section collection and image acquisition method, and is very suitable for popularization and application in electron microscopic imaging or optical microscopic imaging laboratories.