The invention discloses a novel intelligent subway flood-proof protective air-tight door opening and closing control system. The system comprises a control cabinet, an opening and closing device, an electric locking device, a detection device and a subway power supply system, wherein a PLC, a UPS, a switch power supply and a first automatic switching circuit are arranged in the control cabinet; the detection device comprises water level sensors arranged at the lowest position of a protection running tunnel, the water level sensors are in communication connection with the PLC, the PLC is connected with the opening and closing device and the electric locking device separately, and three sets of redundant same-model sensors are used as the water level sensors; signal validity is in a two-out-of-three voting mode, and a PLC program only considers that the water level reaches a preset value when it is detected that water level signals of two water level sensors are valid. According to the change of the water level of a subway tunnel, a flood-proof protective air-tight door is automatically opened and closed so that emergency closing can be carried out when an accident occurs, a river crossing tunnel can be closed, and the safety of equipment, such as trains, in a subway line and the personal safety can be protected.