The invention discloses a dry one-way drawing process of a lithium-ion battery separator, the lithium-ion battery separator and a lithium-ion battery and relates to the technical field of preparation of the lithium-ion battery separator. The dry one-way drawing process of the lithium-ion battery separator comprises the following steps that a polyolefin base film is subjected to pre-drawing before annealing treatment and subjected to secondary drawing after annealing treatment, wherein the drawing multiplying power of pre-drawing is 1.1-1.5 and the drawing temperature is 100-150 DEG C. The problems that as for traditional dry one-way drawing, the lithium-ion battery separator drawing strength and puncture strength are low, the drawing strength is 170-180 MPa at most, and the separator strength requirement cannot be met are solved. According to the separator dry one-way drawing process provided by the invention, as pre-drawing is performed before annealing, the drawing process is performed stage by stage; and by means of the process, the strengths of the lithium-ion battery separator subjected to dry one-way drawing can be improved, specifically, the drawing strength of the separator subjected to dry one-way drawing can be improved by 20-30%, and the puncture strength of the separator subjected to dry one-way drawing can be improved by 10-20%.