The invention provides a texture manufacturing method and a substrate assembly. The texture manufacturing method comprises the following steps: coating a to-be-processed texture region of a substrate with an organic matter so as to form the condition that the substrate and an organic matter layer are overlapped with each other, wherein the organic matter layer is composed of the organic matter that the substrate is coated with; and controlling the laser to irradiate the organic matter layer with a preset rule so as to remove the organic matter irradiated by the laser, and thus forming a texture area on the organic matter layer. The texture manufacturing method does not directly process on the substrate, on the one hand, the integration and the strength of the substrate can be guaranteed; and on the other hand, when a texture needs to be modified, the substrate can be reworked and does not need to be directly discarded, so that waste of materials is avoided, and thus the production cost is reduced. In addition, the organic matter layer that the substrate is coated with is subjected to laser engraving, and the thickness of the required organic matter layer is very small, thus the overall thickness of a finished product is reduced, and the problem that the thickness of the finished product is thicker when a traditional UV transfer printing technology is adopted is avoided.